The Lions defeated the Adelaide Crows 6.2 (38) to 3.2 (20) in front of 22,934 fans at Adelaide Oval.
Emma finished the game on the bench after suffering a hamstring strain and the 32-year-old now plans to retire.
Emma was born and raised in Bundaberg. She grew up a passionate Brisbane Broncos fan and played soccer from the age of 6. She first played Australian rules in 2008 at the age of 19 in Brisbane.
Bundaberg Region Mayor Jack Dempsey congratulated Emma on her success.
“Emma Zielke is a credit to Bundaberg, to her family, her club and to herself,” Mayor Dempsey said.
“Emma is a terrific role model for young girls and I wish her every success in the next phase of her career.”
Brisbane Lions coach Craig Starcevich said Emma would be sorely missed after stamping her name into the Lions' history books.
“She's been captain for over five years, the girls love her, the coaching staff admire everything she's done,” Starcevich told the AFL website.
“She's Brisbane Lions to her back teeth, she's the heart and soul of our football club.”